AP Biology, Chapter 6
Enzyme Simulation: Toothpickase

In your lab notebook: 
	Entered in Table of Contents		2
	Title/Date				2
	Objectives				10
	Procedure				15
	Data					20
	Graph					12
	Conclusion Questions			12

Objectives
	1. Simulate enzyme kinetics.
	2. Test the effect of an enzyme inhibitor on enzyme activity.
	3. Test the effect of temperature on enzyme activity.
Procedure
	Set Up
		1. Choose one member of the group to break.
		2. Hands = toothpickase, fingers used to break = active site, whole toothpick = substrate
		3. Reaction: whole toothpick  2 halves
		4. Starting “solution”: 50 whole toothpicks in a container.
		5. Action: without looking, find, break in half, leave halves in continer
	Part A
		1. Breaker breaks as fast as possible in 10 seconds without looking.
		2. Stop, count the number broken, record.
		3. Break for another 10 seconds; stop, count, record.
		4. Continue through 90 seconds.
	Part B
		1. Starting “solution” = 50 toothpicks + 50 paper clips
		2. Break without looking, stop, record as in Part A
	Part C
		1. Break 10 toothpicks without looking, record the time required
		2. Ice hands
		3. Break 10 fresh toothpicks without looking, record the time required
Data
	Part A: table; 	Title
			Time :0, 10 sec, 20 sec …. 90 sec
			Number broken in interval
			Total broken from 0 sec
	Part B: SAME
	Part C: table	Title
			Temperature: room temperature, iced
			Time to break 10 toothpicks
Graph
	Both Part A and B on the same graph
	Both Time vs. Total Broken
	Add title, label axes, add legend
Conclusion Questions
	1. How does the number of toothpicks broken change over time?
	2. Write a sentence to describe how mixing in another substrate affects how fast an enzyme works.
	3. Write a sentence to describe how temperature affects how fast an enzyme works.