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The value of the Wright-Giemsa stain for diagnosing hairy cell leukemia in body cavity fluids.
Strobel SL, Brandt JT.
We compared the value of the Wright-Giemsa stain with the Papanicolaou stain for diagnosing hairy cell leukemia in the body cavity fluids of three patients. The cytological features of the leukemic cells were much clearer on the Wright-Giemsa-stained preparations. This allowed for the accurate diagnosis of leukemic involvement of the body cavity fluids in 3/3 patients using the Wright-Giemsa stain but only 1/3 using the Papanicolaou stain. A tartrate-resistant acid phosphatase stain was used to confirm the diagnosis in each case.
PMID: 2430142 [PubMed - indexed for MEDLINE]
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Hairy cell leukemia (leukemic reticuloendotheliosis) in serous effusions.
Krause JR, Dekker A.
The cytology of two cases of hairy cell leukemia (leukemic reticuloendotheliosis) involving pleural and ascitic fluids is described. With the Papanicolaou stain, the cells have a "lymphoid" appearance. The presence of these cells plus knowledge of the clinical history might help the cytologist and/or hematologist in their identification. A tartrate resistant acid phosphatase (TRAP) stain will confirm the true nature of such cells and establish the clinical diagnosis of hairy cell leukemia. Unlike the other members of the leukemia-malignant lymphoma group, the body cavities are rarely involved.
Publication Types:
PMID: 78614 [PubMed - indexed for MEDLINE]
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The influence of Romanowsky-Giemsa type stains on nuclear and cytoplasmic features of cytological specimens.
Schulte E, Wittekind D.
Anatomisches Institut II, Freiburg, FRG.
The aim of the present study was to compare the staining pattern of the standard azure B-eosin Y stain with commercial May-Grunwald-Giemsa (MGG) stains on cytological specimens by means of high resolution image analysis. Several cytological specimens (blood smears, abdominal serous effusions, bronchial scrape material) were air dried, methanol fixed and stained with the standard azure B-eosin Y stain and with commercial May-Grunwald-Giemsa stains. Integrated optical density (IOD) and colour intensities of cell nuclei and cytoplasm were measured with the IBAS 2000 image analyser. Commercial MGG stains gave much higher coefficients of variation for all parameters than the standard stain. Reproducibility of cell nuclei segmentation versus cytoplasm was significantly better for the standard stain. Contamination of the standard stain with methylene blue partly copied the staining pattern of commercial stains. The standard azure B-eosin Y stain is recommended for high resolution image analysis (HRIA) of cytological samples.
PMID: 2484852 [PubMed - indexed for MEDLINE]
Chylothorax in chronic lymphocytic leukemia patient.
Doerr CH, Staats BA, Markovic SN.
Division of Pulmonary and Critical Care Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA. [email protected]
Chronic lymphocytic leukemia (CLL) is rarely complicated by chylothorax: we present a 93-year-old woman with CLL who developed recurrent pleural effusions that were ultimately found to be chylous in nature. Despite eight repeated thoracenteses, she continued to experience re-accumulation of fluid, and therefore, video-assisted thoracotomy with mass ligation of the thoracic duct region plus pleurodesis was performed to resolve the chylothorax. Despite her age and underlying disease, she did well during follow-up. The etiology and management of chylothorax are also reviewed. Copyright 2002 Wiley-Liss, Inc.
Publication Types:
PMID: 12111770 [PubMed - indexed for MEDLINE]
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Adult T-cell leukemia/lymphoma: a cytopathologic, immunocytochemical, and flow cytometric study.
Dahmoush L, Hijazi Y, Barnes E, Stetler-Stevenson M, Abati A.
National Institutes of Health/National Cancer Institute, Section of Cytopathology, Bethesda, Maryland 20892, USA.
BACKGROUND: Adult T-cell leukemia/lymphoma (ATLL) is a postthymic lymphoproliferative neoplasm of T cells caused by human T-cell lymphotropic virus (HTLV-1). Most cases are found in Japan, the Caribbean basin, and West Africa. DESIGN: To identify diagnostic parameters for cytology in this neoplasm, the authors undertook a retrospective review of all ATLL samples from 1990 to 2000. RESULTS: One hundred fourteen samples from 34 patients with the diagnosis of ATLL were reviewed: 80 cerebrospinal fluids, 7 pleural effusions, 4 bronchoalveolar lavages, 2 peritoneal effusions as well as fine-needle aspirations of 15 lymph nodes, 4 subcutaneous lesions, and 2 breast nodules. Twenty-one patients were women and 13 were men, with an age range of 30 to 71 years. Morphologically, all specimens were characterized by the presence of a polymorphous population of lymphocytes ranging from small bland-appearing lymphocytes to large atypical ones with bizarre, multilobulated nuclei (flower-like or clover leaf cells) with coarse chromatin and prominent nucleoli. The cytoplasm was deeply basophilic with occasional vacuoles. Immunocytochemistry was performed on 17 specimens from 14 patients. In all cases tested, tumor cells were immunoreactive for CD3, CD4, CD5, and CD25 and were nonimmunoreactive for CD7 and CD8. Flow cytometry was performed on 12 specimens from 9 patients. The tumor cells in all cases tested were positive for CD2, CD3, CD4, CD5, and CD25 and were negative for CD7. CONCLUSIONS: Despite the polymorphous nature of ATLL, diagnosis can be established by close attention to nuclear cytologic features in conjunction with ancillary studies such as immunocytochemistry and/or flow cytometry. Copyright 2002 American Cancer Society.
PMID: 11954028 [PubMed - indexed for MEDLINE]
Pleural effusion in chronic myelomonocytic leukemia.
Bourantas KL, Tsiara S, Panteli A, Milionis C, Christou L.
Department of Internal Medicine, University of Ioannina Medical School, Greece.
Pleural effusion in 4 patients with chronic myelomonocytic leukemia (CMML) is described in this report. According to the literature, pleural effusion in CMML is a poorly understood and rare occurrence. Two of our patients presented with pleural effusion as an initial symptom while the other 2 developed it during the course of the disease. In only 1 patient was the pleural effusion due to leukemic infiltration while in the other 3 it was a reactive phenomenon. Peripheral lymphadenopathy was observed only in the former patient who died of acute leukemia. After prednisolone therapy the pleural effusions resolved in the other 3 patients.
Publication Types:
PMID: 9490564 [PubMed - indexed for MEDLINE]
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