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Abstract |
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Immunohistochemical Study of Metallothionein Isoform II in Hyperplastic,Dysplastic and Neoplastic Prostatic Lesions Sonia
L. El Sharkawy; Marwa A. El Shaer; Manal A. Badawi and
Mohamed H. kobeisy* Pathology
Department, National Research Centre; *Pathology
Department, National Institute of Nephrology and Urology. Abstract
Metallothioneins (MTs) constitute a family of several intracellular,
low molecular-weight proteins with a high affinity to various heavy metals.
They are involved in metalloregulatory functions such as cell proliferation,
growth, and differentiation. The goals of the present study were to
investigate the expression of MT in hyperplastic, dysplastic and neoplastic
prostatic lesions and to correlate its expression with histologic grade of
prostatic carcinoma.
This study was performed on Formalin fixed and paraffin embedded tissue
blocks from 8 patients with benign prostatic hyperplasia (BPH), 6 patients
with prostatic intraepithelial neoplasia (PIN) and 30 patients with prostatic
carcinoma (PC) using streptavidin-biotin technique. The histologic grade was
defined and the carcinoma cases were divided into 8 low grade (Gleason 2-4),
12 moderate grade (Gleason 5-6) and 10 high grade (Gleason 7-10) carcinoma.
Normal and benign prostatic tissues
showed patchy MT staining of epithelial cells. All cases of PIN, 20 out of 30
PC cases (66.7%) showed positive staining for MT. MT expression significantly
increased from low to high grade tumours. The proportion of MT positively
stained cells was directly correlated with histologic grade of PC. The
epithelial cells lack uniformity in staining intensity, but the percentage of
strongly positive cells increased with increasing the histologic grade of PC.
The high incidence of MT expression in PIN in this study suggests that
it is associated with early prostate tumorigenesis. Also MT expression was
directly correlated with histologic grade of PC suggesting that MT may be a
useful marker for predicting prognosis of cancer prostate. |
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