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Abstract |
Active Immunization Of Rabbit With Gamma Irradiated Cobra
(Naja
haje) Venom Toxoid
Department
of Drug Radiation Research and Technology,
Atomic
Energy Authority
Cobra (Naja haje) venom detoxified by gamma radiation (15KGy or
25KGy) was used as toxoid
for active immunization of rabbits following a short schedule ( on day 0,
7,21) of immunization with complete Freund's adjuvant. Effective neutralization
of venom toxin by immune sera of rabbits was observed. The presence of antibody
in the immune sera was detected by immuno-diffusion test. The non-irradiated and
the two dose levels gamma irradiated Naja haje venom, against the
antivenin antibody produced with non-irradiated venom or against antivenin
antibody produced with 15 KGy gamma irradiated venom all showed similar
patterns. Also there was no change in the titer of antivenin solution obtained
from the rabbit immunized with 15KGy irradiated venom , as compared with that
antivenin solution obtained from rabbit immunized with non-irradiated venom.
Sera of rabbits immunized with 15KGy irradiated venom toxoid (15KGy toxoid
antiserum) neutralized venom lethality, 95% of protease activity and 50% of
phospholipase A2.
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