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Abstract |
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The Possible Ameliorative Effect Of Dimethyl Dicarboxylate Biphenyl (DDB) Against Mancozeb–Induced Testicular Toxicity In Rats Manar Effat SelimZoology Department, Faculty
of Science, Ain Shams University Abstract Mancozeb, an
organocarbamate fungicide, has been widely used for several decades. Despite
the wide scope of exposure to this compound, up till now, there is a scarcity
in published studies on the toxicity of mancozeb and its impacts on cell
structure and functions. Thus the present study was designed to investigate
the possible counteracting effect of dimethyl dicarboxylate biphenyl (DDB) in
reducing the hazards caused by mancozeb in the testies. The effects of
mancozeb given separately or simultaneously with DDB on rat testis were
studied histologically and ultra structurally. Twenty-one male albino rats
were divided into three groups, 7 rats each. The first group was given orally
0.5 ml of distilled water and served as control. Animals of the second group
were given mancozeb orally at a dose level of 500 mg/kg b.wt for 15
consecutive days. Animals of the third group received the same dose of
mancozeb as in the second group and simultaneously given DDB (50 mg/kg b.wt)
through the same route of administration. Mancozeb treatment induced cellular
damage manifested as exfoliation and destruction of the spermatogenic lineage.
Destructed spermatids and Leydig cells were also pronounced. The ultra
structural observations revealed marked cytoplasmic vacuolation in Sertoli
cells and spermatogonia. Some cytoplasmic organelles of the spermatogonia and
primary spermatocytes were degenerated. Moreover, destructed spermatids were
pronounced in most of the seminiferous tubules. The interstitial cells
appeared devoid of lipid droplets and the mitochondria appeared with
disintegrated cristae. Administration of
DDB simultaneously with mancozeb ameliorated most of the testicular tissue
damage. This was reflected as restoration of the normal appearance of the
spermatogenic lineage. Numerous spermatids were demonstrated in the lumens of
the seminiferous tubules, most of them showed similar structure to those of
the control group. In conclusion, it is possible
to suggest that DDB might have ameliorated and amended the abnormalities
caused by mancozeb.
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