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Abstract |
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L -Carnitine and Melatonin Reverse CCl4
Induced Liver Fibrosis In Rats (Histological and Histochemical Studies) Fatma,
A. Morsy, Abdel Razik, H. Farrag and Sonya, L. El-Sharkawy
Abstract
Carbontetrachloride (CCl4) is closely related chemically to
chloroform and likewise in hepatic poisons. This study was designed to
evaluate the effects of carbon tetrachloride on liver of male rats and the
reversing effects of L-carnitine and melatonin on established liver fibrosis.
A total of 72 adult male albino rats were used in this study. The animals
were divided into six groups. Group (1) animals of the first group were kept
as control andtreated with paraffin oil twice weekly for eight weeks. Group
(2) rats of the second group were injected with CCl4 intraperitoneally
at 0.15 ml per rats (diluted 1:1 in liquid paraffin) twice weekly for eight
weeks to produced liver fibrosis. Group (3) following establishment with CCl4
which induced liver fibrosis, the rats were treated with L-carnitine at a
dose level of 50 mg/kg for four weeks. Group (4) rats with liver fibrosis
were injected intraperitoneally with melatonin at dose level of 10 mg/kg for
four weeks. The fifth and sixth groups were given L-carnitine and/or
melatonin at dose levels of 50 mg/kg and 10 mg/kg respectively for four
weeks.
Histological changes in the liver of rats treated with CCl4
including liver fibrosis, architecture distortion and appearance of many
pseudolobule. The fibrous tissues run in septa between the nodules. The liver
damage varied from one area to another and varied from moderate fibrosis to
cirrhosis.
Quantitative measurement of the severity of liver fibrosis (area
damage) was achieved by using computerized image analysis (Leica image)
showed that highly significant increase in area of fibrosis was recorded in
the case of rats treated with CCl4 only.
Quantitative DNA image analysis showed that 3% of aneuploid cells
could be noticed in liver of rats treated with CCl4 only.
Histochemical results of rats treated with CCl4 showed highly
significant increase in grey level of mucopolysaccharides and protein levels.
No histological and histochemical changes could be noticed in the liver of
rats treated with either L-carnitine or melatonin only. Both L–carnitine and
melatonin were found to reverse CCl4 induced liver damage. |
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