|
Abstract |
|
Ultrastructural study of renal tubular damage induced by captopril
in adult and fetal mice Hamdy H. Swelim and Aleya
A.Sakr Department of
Zoology, Faculty of Science, Abstract
The present study has been designed to evaluate the possible nephrotoxicity of the angiotensin
converting enzyme inhibitor, captopril on renal
tubules of adult and maternally treated fetuses of CD-1 mice. The study
included the effect of captopril administration for
one month up to three months in adults, while in fetuses,
they were exposed to the drug through their mothers in two periods. The first
was from 6th-12th days of pregnancy, while the second
was from 6th -18th
day of pregnancy. The dose used in the present study represents the
dose equivalent to the
therapeutic daily dose taken by human. All the recorded tissue
damage was found to be time dependent. The first remarkable feature noticed
in all the treated adult animals was the presence of hyaline casts that
obstructed most of the renal tubules. The second remarkable feature was the
increase of the intertubular space associated with
irregularity of the tubules due to the degeneration and vacuolation
of the basal regions of the cells. Renal tubule cells showed large blebs, accumulaton of lipids, degeneration and necrosis. In
maternally treated fetuses,
the proximal convoluted tubule cells displayed moderate vacuolation and marked increase of lysosomes
while some of the distal convoluted tubules revealed atrophy and their cells
showed loss of mitochondria. In addition, the collecting tubules showed loss of microprojections. Worthy to mention that there was
apparent increase of mesenchymal cells as well as
fibroblasts in the fetuses maternally treated with captopril.
The significance of these changes was discussed and it should be emphasized
that captopril must be taken with caution for
pregnant women and those who suffer from renal troubles. Moreover, kidney
function should be monitored during therapy . |
|
|