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Abstract |
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Immunohistochemical Study Of Bcl-2 Protein And Estrogen Receptor-Alpha Expression In Benign Prostatic
Hyperplasia And Prostatic Carcinoma Ahmed H. Abel-Rahman- Ghada A. Abdel-Aziz*- Ali Emad
S**ــ Abdel-Basset A.
Badawy***- Alaa Ar. Abdel-Hafez*** Departments of Pathology, Dermatology &
Veneriology* and Urology** Al-Azhar University (Assiut & Girls) and South
Valley University*** Abstract:
The human prostate, a male sexual accessory tissue involved in seminal
fluid production, has a remarkably high incidence of hyperplastic and
neoplastic disease. The present study was carried out on one hundred and
twenty (120) specimens divided into two groups; group 1: Included forty cases
of benign prostatic hyperplasia (BPH) and group 2: Included sixty cases of
prostatic adenocarcinoma (PC) (22 were low grade; GS: 2-6 and 38 were high
grade; GS: 7-10),in addition to twenty cases of histologically normal
prostates taken as controls. Immunohistochemical technique was applied to
detect Bcl-2 as well as ER α positivity in all specimens. Group 1 showed the
following profile: ER α (+) in all
cases (100%), Bcl-2 (-) in 95%, ERα (+) /
Bcl-2 (+) in 95%, ERα (-) / Bcl-2 (+) in 0%, ERα (+) / Bcl-2 (-) in 5% and ERα (-) / Bcl-2 (-) in 0% of cases while group 2 showed
the following profile: ERα (+) in
30%, Bcl-2 (+) in 21.7%, ERα (+) /
Bcl-2 (+) in 15%, ERα (-) / Bcl-2 (+) in 6.7%, ERα (+) / Bcl-2 (-) in 15% and ERα (-) / Bcl-2 (-) in 70% of cases. The mean
epithelial ER α -immunolabeling was,
however, significantly increased in group 2 than in group 1 (P < 0.05)
which, in turn, being higher than the normal cases (P<0.05 ) . Among
group 2 , the mean ER α immunolabeling was
significantly more in high grade than in low grade tumors (P < 0.05), however, the mean ER α
immunolabeling revealed no significant correlation with T-stage (P =
0.219) or with the clinical stage (P = 0.391). In contrast, the Bcl-2
immunostaining was statistically higher in group 1 than in group 2 (P < 0.05)
and showed a significant correlation with T stage (P < 0.05) although the
study displayed no significant correlation between Bcl-2 immunopositivity and
either Gleason score (P = 0.125) or the histologic grade (P = 0.146). In
addition, combined ER α (+)/ Bcl 2 (+) immunoreactivity demonstrated
the aggressive subgroup of PC cases more accurately than either ER α (+) or Bcl-2
(+) alone. Finally, multivariate analysis showed that the Bcl-2, proved to be
an independent prognostic indicator (P < 0.05). Thus, the
immunohistochemical expression of ER α and Bcl-2 protein in prostatic tissue may
aid in better understanding the biology and genesis of both prostatic
hyperplasia and carcinoma . |
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