MICHAEL (HONGWEI) SUN

 

 

SUMMARY

     Bioinformatics Skills:

Working experiences in genomic databases, genomic annotation, sequence comparison, sequence alignments, gene analysis and expression, gene sequencing, and statistical analysis.  Experiences in physical mapping and sequence assembly, localizing RefSNPs, and predicting protein domains and general structures

Computer Skills:

Experiences with full life cycle development.  Two years solid working experiences with Java2, including Applet, GUI design, AWT, Swing, 3-tier Applications, Servlet, JDBC, EJBs, as well as perl and Visual Basic.  Experiences in web development include ASP, JSP, XML, HTML, MS Interdev, and Javascript.  Data base management skills include two years’ working experiences in MS SQL Server7.0/2000, Oracle Certified SQL and PL/SQL Developer Oracle8 &8I,  MS Access. 

    

     Biology Skills: 

           

cDNA library construction, gene cloning, DNA and RNA isolation, DNA sequencing, PCR, site-directed mutagenesis, Southern blot, Northern blot, Western blot, protein expression and purification from insect cell (SF21), E. coli and mammalian cells, post-translational modifications, RNA transcription, ligand–receptor binding assays, screening ligands interacting with ligand gated ion channels, drug transport assay with cell membrane, and biophysical recording.

  

EDUCATION

Web Application Development (Microsoft Endorsed) (80 hours), Arthur Andersen, Houston, July 2000

Savvion Business Manager for Application Development (Savvion Endorsed) (40 hours), Houston, June 2000

Java [tm] Programming Language Workshop (Sun endorsed) ( 40 hours), Houston, February 2000

Advanced Java Project Class ( 76 hours), Fastrain Computer Training Center, Houston, January 2000

Extensive Training for Sun Java2 Programmer Certification (160 hours), Mo’s Computech, Houston, August 2000

 Ph.D. (Genetics): Virginia Polytechnic Institute and State University (Virginia Tech), December 1996

                  

M. S. (Genetics): Chinese Academy of Agricultural Sciences, Beijing, China,    August 1985

 

B. S. (Agronomy): Agriculture University of Hebei, China, August 1982

 

CERTIFICATES

·        Sun Java2 Certified Programmer (SJCP)

·        SQL and PL/SQL (Oracle Certified)

·        Develop PL/SQL Program Units (Oracle Certified) 

·         A Recipient of the AACR (American Association for Cancer Research) Glaxo               Wellcome Oncology Clinical Research Scholar Award.  February 1997

 

WORKING EXPERIENCES

June 2001 – present  Bioinformatician, Human Genetics Center, Univ. of Texas, Houston.  Projects participated include:

·      Automatic Transformation of Biological Data (VB, VBA)

·      Characterization of DNA Sequences (Perl, Celera)

·      Data Process of DNA Samples in Freezers (SQL Server, Access Project)

·      Critics on the Celera Gene Discovery System (Celera Discovery System))

·      Establishment and management of SNP Database (SQL Server, VB, Access)

June 2000 – June 2001  Java Programmer,  Arthur Andersen LLP, Houston.  Projects participated include:

·        Andersen TTC Web Development for Client Satisfaction Survey (Ms. Visual Studio, JavaScript, ASP, and HTML)

·        Andersen TTC Web Development for Staffing Assignments ((Ms. Visual Studio, JavaScript, ASP, and HTML))

·        New Business Process (Database Setup, Adapter Coding with Java, and Integration)

·        Servlets Manipulating XML_Files (Java Servlet and XML)

·        Applets Manipulating Files (Java Applets)

·        Broker Tool (3-tiers) (Java2)

·        RD Data Correction (Java Programming)

·        TTC Network Infrastructure

·      Channel One-Data Warehouse

June 1999 – June. 2000 Programmer/Analyst, Sunrise Biotech Inc., Houston.  Project: - PDM Data Management System for Sunrise Biotech Inc. The www based user interface provides a data management tool for the PDM process.

·        Established a functional personnel database with oracle.

·        Established a functional product database with oracle.

·        Developed a homepage for the company with HTML, JSP, and JAVA. The homepage         will be linked to the databases established with JDBC.

June 98 – June. 99 Research Faculty of Instructor, Baylor College of Medicine, Houston. Investigating the mechanism of nicotine actions at the nicotinic receptor; developing and implementing data acquisition and analysis software with Java, and Fortran; computer modeling of protein; image processing with scientific (ImageJ, MetaMorph, FluoView), Adobe(Photoshop, Illustrator) and CorelDraw graphic software.

June 94 – June 98 Research Associate, Texas Tech University, Lubbock.  Investigating the drug action sites of brain  receptors.

Aug. 90 – June 94 Research Associate . Virginia Tech, Blacksburg. Biological data analysis.

MANAGERIAL AND ADMINISTRATIVE SKILLS

• Technical training of junior programmers and laboratory personnel.
• Experiences in presenting at interpersonal skills.
• Upbeat attitude and rapid response to fast pacing environment.
• Writing of software documentations, grant proposals and research articles.

PUBLICATIONS:

 

Papers:

 

1).  Sun, H. and Schmidt, R. E. 1995.  Alteration  of Protein Syntheses in Bentgrass Plants under Seaweed Extract Applications.  HortScience 3: 199-211.

 

2). Sun, H., Zhu, Q., and Center, M.S. 1996.  Expression and Characterization of the Multidrug Resistance-associated Protein in Insect Cells Infected with Recombinant Baculovirus.  Oncology Research 8: 287-293.

 

3). Tanowitz, M., Sun, H., and Mei, L. 1996.  Surgical Denervation Increases Protein Tyrosine Phosphatase Activity in Skeletal Muscle.  Brain Research 712: 299 - 306. 

 

4). Sun, H., Schmidt R.E., and Eisenback, J.D. 1997. The Effect of Seaweed Concentration on the Growth of Nematode-infected Bentgrass Grown under Low Soil Moisture. Eighth International Turfgrass Research Conference Journal. Sydney, Australia. 8:1336-42.

 

5).  Center, M. S., Zhu, Q., and Sun, H. 1997. Multidrug Resistances Conferred by Non-P-Glycoproteins. Drug Resistance 12: 1278- 1301.

 

6).  Zhu Q., Sun, H., and Center, M. S. 1997. Functional Analysis of the Nucleotide Binding Domains of the Multidrug Resistance Protein (MRP). Oncology Research 9: 229 -  236.

 

7).  Center, M. S., Zhu, Q., and Sun, H. 1998. Mutagenesis of the Putative Nucleotide Binding Domains of the Multidrug Rsistance Protein (MRP). Analysis of the Effect of These Mutations on MRP Medited Drug Resistance and Transport. Cytotechnology 27: 61-69.

 

8).  Sun, H., McCardy, E. A., Machu, T.K., and Blanton, M.P. 1999.  Characterizing the Interaction of 3,4,5-trimethoxybenzoic Acid 8- (dietylamino) Octyl Ester (TMB-8) with the Torpedo Californica Nicotinic Acetycholine Receptor and the 5-HT3 Receptor. Journal of Pharmacology and Experimental Therapeutics 290:129-135.

 

9).  Coultrap, S.J., Sun, H., Tenner, T.E., and Machu, T.K.  1999. Competitive Antagonism of the Mouse 5-HT3 Receptor by Bisindolylmaleimide I, a Selective Protein Kinase C Inhibitor. Journal of Pharmacology and Experimental Therapeutics 290:76-82.

 

10). Sun, H and Tina K. Machu.  2000. Bicuculline Competitively Antagonizes 5-Hydroxytrptamine₃ and Glycine Receptors. European Journal of Pharmacology : 391:243-249.

 

11). Sun, H, Steven J. Coultrap, and Tina K. Machu. 2000. Chimeras of Mouse and Human 5-Hydroxytrptamine3 Receptors reveal a Domain Responsible for Curare Sensitivity.  Neuropharmacology (submitted).

 

12). Tina K. Machu, Margaret E. Hamilton, Tonia F. Frye, Christopher L. Shanklin, Michael C. Harris, Hongwei Sun, Thomas E. Tenner, Jr., Ferenc S. Soti, and William R. Kem

Benzylidene Analogs of Anabaseine Display Partial Agonist and Antagonist Properties at the Mouse 5-Hydroxytryptamine_3A Receptor  J Pharmacol Exp Ther 2001 299: 1112-1119.

 

13). Sun, H and Tina K. Machu. Identification of Two Novel Curare Sensitive Sites of 5-    Hydroxytryptamine₃ Receptors (5-HT₃R) by Constructing Chimeric Receptors and Site-Directed Mutagenesis. Journal of Biological Chemistry (submitted).

 

14). Sun, H and Tina K. Machu. Characterization and Localization of Two Novel Positive Allosteric Sites by Colchicine on Human 5-Hydroxytryptamine₃ Receptors (5-HT₃R) by Constructing Chimeric Receptors and Site-Directed Mutagenesis. (Manuscript in Preparation).

 

 

Abstracts and Presentations:

 

1).  Tanowitz, M.B., Sun, H.W.,  and Mei, L. 1995. Tyrosine Phosphatase Activity in Rat Lower Leg Muscles Increases Following Sciatic Nerve Transection. Soc. Neurosci. Abstr., Vol 21, Part 1 243.10.

2).  Won, S., Si, J., Sun, H., and Mei, L. 1997. A Single Amino Acid Switch Increases the Catalytic Activity of a Novel Brain-enriched Protein Tyrosine Phosphatase.  Soc. Neurosci. Abstr., Vol 23, Part 1 277.5.

3).  Sun, H., Zhu, Q., and Center, M. S. 1997.  Mutagenesis of Putative Nucleotide-binding Domains of Multidrug Resistance Associated Protein (MRP): Analysis of the Effect of these Mutations on MRP Substrate Transport.  Annual Meeting of American Association for Cancer Research. San Diego, California.

4).  Zhu Q., Sun, H., and Center, M. S. 1997. Functional Analysis of the Nucleotide Binding Domains of the Multidrug Resistance Protein (MRP).  Annual Meeting of American Association for Cancer Research. San Diego, California.

5). Sun, H., Coultrap, S.J., and Machu, T.K. 1999. Chimeras of Mouse and Human 5-Hydroxytryptamine₃ Receptor Reveal Two Domains Responsible for Curare Sensitivity. Soc. Biophysics. Baltimore, Maryland.

6). Tarbox, J.A., Sun, H., Field, T.R., and Machu, T.K. 1999. Characterization of a Residue on TM2 Region of the Mouse 5-HT₃ Receptor Responsible for Alcohol Sensitivity. Student Research Day, Texas Tech University Health Sciences Center (Third Prize).

7). Tarbox, J.A., Sun, H., Field, T.R., and Machu, T.K. 1999. Mutation of Gly288Ser in the 5-HT₃ Receptor Enhances Alcohol Sensitivity. 1999 TRSA Scientific Meeting. Dallas, Texas.