The Iron Ladies is the name of the team of the transvestites and transsexuals who became famous in Thailand for winning the national men's volleyball championships. However, on one gloomy day after their win, the team splits into two. Joining the new team is April, May and June as well as Nhong, the gigantic gay guy who is Nong's new buddy. Meanwhile the original team seems to have lost its touch and suffers a series of defeats. In the national Thailand league, Nong's team goes head to head against the original Iron Ladies team and beats them three times. Nong argues incessantly with Jung; the two former best friends are now bitter enemies, hurling insults at each other. Jung and Chai decide to make a journey to Xishung Bania in China to Pia, the beauty queen who's now making a career out of being a showgirl.

It is not just a long rough trip to meet a friend. It's also full of sweet, spicy and hilarious memories of the early years when they first met at university. Each of the members relates stories about how they first met, how they got to know one another and how they formed the volleyball team. They also reveal how Jung became gay over marring his girlfriend. And, of course, there are the wonderful memories of victory as a volleyball team.

In Bangkok, Nong is hurt when he finds out Kitti has appointed Nong's arched-enemy and hater of gays, Muangman, as the team leader. Nong asks Mon to take him to Xishuang Banla in China to visit Pai. Now everyone, Jung and Chai as well as Mon, Nong, April, may and June are traveling to Xishuang Banla? And there is an added surprise because Wit and Coach Bee are also there! It is then they reach the understanding that Pia has planned to get all the former friends together again.

In Xishuang Banla, the Iron Ladies are invited to a match with the local team. Old wounds are healed, and the magic of the Iron Ladies returns. The crowd goes wild. Later the team goes back to Lampang, where their story first begin, in order to become the representative team for District 5…while on the other side of the net is their eternal enemy Muangman.