| Antibiotic Resistance |
3) Finally, read the review of the original paper (Tie me up, Tie me down: Inhibiting RNA polymerase) and answer the following questions briefly. Come to class tomorrow with any questions you have about this paper. a) Explain how a search for resistant mutants can give information as to the antibiotic�s target and mechanism of action. b) By adding the antibiotic (myx) at different times in a transcription reaction, the authors showed that myx inhibits RNA polymerase binding to double stranded DNA. Explain. |
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