blood and tissue nematodes (round worms) Wuchereria bancrofti Brugia malayi [Malayan filariasis] Loa Loa [African eye worm] Onchocerea volvulus (convoluted microfilaria) Dracunculosis ,Medina worm ,Guinea worm
habit lymph vesels and glands of man lymphatics above diaphragm SC tissue (free migrating)  , appear under conjunctiva skin (encapsulated in fibrous nodules) in SC tissue cutaneous and SC tissue of man and animals
microfilaria *loose sheath *free from nuclei *loose sheath e 2 distinct nuclei in tail tight sheath ,full of nuclei no sheath *tail free from nuclei *adult worm e triangular mouth ,with 6 papillae around it *gravid female uterus show huge tube ,ovary packed e larva *** D.H >man ,dog ,monkey ,horse ,cattle *** I.H >cyclops ,infective stg :larva in cyclops
periodicity nocturnal non periodicity (nocturnal) diurenal periodicity (in blood) non periodicic (in skin & SC)
vector culex  Aedes ,Anopheles mansonioides ,Aedes ,Anopheles chysops (horse fly)  
pathogenicity *due to adult worm (microfilariae may cause slight or no pathogenicity) >inf lesions ,cellular infilteration ,eosinophilia >fibrosis of lymphatics >varricose *** ELAPHANTIASIS : as result of continous infection affect legs & genitalia where marked growth of fibrous tissue around dead worms also marked hypertrophy of skin. and migrate from place to place in SC under skin  ,,(appear under conjunctiva ,it may go from one eye to another through bridge of nose) *both adult worms & microfilaria are found in in skin but not in blood * sc tissue usu encapsulated in fibrous nodules in any part of body ,*in african :lesions in trunk things and arms *in american : :head and shoulders lesions are most seriuos and responsible for much blindness when worm reach skin >liberate toxic substance >blister is fromed symptoms :allergic Rx due to rupture of blister (appear during operative removal of worms or when it broken during extn and larva escape to Sc tissue ** 2ry bacterial infection may result in abscess formatn
diagnosis 1-exam of blood (taken at night for microfilaria ) 2-serological test a-intradermal skin test using filarial ext as antigen b- ELISA 3-Hetrazan provocation give 100 mg (small dose) >micro fil appear in blood ein 45 m ,so facilitate diagnosis at any time of day 4-X ray examn for calcified adult worms in lymphatics   *microfilaria demonstratn in aspirate of nodules or skin snips and adult in excised nodule 1-demonstratn adult worm under skin 2-X ray examinatn of dead calcified worms 3-demonstratn of larvae in blisters or in water after their discharge 4-I.D test using antigenic ext of worm 5-complement fixatn test 6-eosinophilia (10%)
distribution topical & subtropical countries far east tropical africa   Egypt ,sudan ,india ,persia ,china ,Brazil ,west Africa
ttt 1-DEC (only ttt fro elephantiasis) given e antihistaminics or steroids 2-antibacterial drugs (for ttt of 2ry infections) 1-surgical removal of adults seen under conjunctiva 2-DEC 2mg/kg 3 times daily for 2-3 weeks e antihistaminics 1-surgical removel of nodule (under anaethesia) 2-DEC (hetrazan) active # microfil 3-suramin (germanin) active # adults 1-rolling up the worm by stick , remove few cms every day (in severe inf  occur if rupture of worm during operatnthus surgical removal by multible incisions after localizatn of worm by X ray) 2-Thiabendazole 3-DEC (hetrazan) and suramin
life cycle adult (lymph vessels) >microfilaria in blood >ingested by mosquito >develop till become infective larva in labium {infective stg} >bit healthy man >larva migrate to lymphatics where it develops to mature worm in D.H >gravid female migrate in SC tissue at w it will be in contact e water (feet ,shoulder ,legs ,arms) >papule >vesicle >ulcer >in contact e water >larva discharge  in water >ingested by cyclops (I.H) (water flea) inside its body cavity(metamorphose in body cavity into infective form )>ingestion of cyclops in well water >larvae release in intestine >migrate to retroperitoneal tissue where they mature
notes larva migrans:migratn of larval nematodes in unsuitable host >larva is unable to complete normal development so distributed in tissues and remain alive for some weeks or even months >dieand destroyed by cells of host in man : 1)cutaneous larva misrans (creeping eruption) > from contact of larvae of dog and cat hookworms (Ankylostoma braziliense or A caninum) 2)Visceral larvae migrans :from contact e toxocara larvae ,that enter through intestinal tract
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