Nucleotide Excision Repair (NER)-
operates by a cut and patch mechanism that removes a variety of bulky lesions,
including pyrimidine dimmers and nucleotides to which various chemical groups
have become attached.
NER
involves in the following steps:
1. Damage recognition
2. Binding of multi-protein
complex at the damaged site
3. Double incision of the
damaged strand several nucleotides away from the damaged site, on both the 5’
and 3’ sides
4. Removal of the
damage-containing oligonucleotide from between the two nicks
5. Filling in of the resulting
gap by a DNA polymerase
6. Ligation