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(Pakistan Science Foundation sponsored under Career Development of Young Scientists and Technologist)

The project was initiated to improve the goat farming through establishment of artificial insemination facilities, documentation of goats farming systems and production of bucks, in the province. The animals were purchased and stationed at animal sheds of Veterinary Research Institute Peshawar. Criteria for selection were freedom from apparent diseases or any reproductive abnormality and representation of the local dairy breeds. After training in semen collection through artificial vagina, the bucks were shifted to individual pens and isolated from the rest of the flock and visitors.

            Semen was collected in an artificial vagina and volume was read at the graduation of the semen collection tube, ignoring the frothy part of the ejaculate. A drop of semen was collected, placed on a slide and examined for mass motility (wave motion). For determining concentration of spermatozoa 50 micro-liters semen was put into 1950 micro liters of spermicidal solution and concentration were worked out adjusting the dilution factor, cell counter in the five bigger cells of the hemocytometer (E1 to E 5) and volume of the solution in the chamber of the hemocytometer. Percentage of living spermatozoa and morphological abnormalities were determined through making smears of semen using nigrosin-eosin stains. Individual motility of spermatozoa was recorded. The semen volume (1.2 to 2.5 ml), and concentration (288 to 1738 m/ml) are sufficient to produce a significant number of doses for use in the field. The semen extender 1 selected for our studies gave a significant protection to maintain the spermatozoa in a viable state for up to 192 hours post-collection.

Fertility trials under field conditions resulted in getting 86.5% conception rate. Ultrasonography was used as a successful tool for diagnosing pregnancy and presence of twins after 30 days of breeding. The method was found as cost effective and feasible under field conditions.

Prof.Dr.M.Subhan Qureshi

Principal Investigator

drmsqureshi@yahoo.com