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DAVID
ERHO
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| Objective: | |
| To apply my experience in research, cGMP, and missions work towards improving people's lives. | |
| Education: | |
| Seattle Pacific University (1995-1999) BS Biochemistry, G.P.A. 3.64/4.00 |
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| Skills: | |
| 3 1/2 years in raw materials cGMP operations Experience with HPLC, FTIR, titrimeters, pH testing, osmometers, flame ID analysis, chemical ID, melting point determination, automated/manual loss on drying, gel electrophoresis, protein expression and purification, sub-cloning, crystallization, and x-ray analysis of crystallized proteins. Working knowledge of SAP, MS Word, MS Access, MS Excel, MS Foxpro, UNIX, LIMS, and AutoCad. |
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| Work Experience: | |
| Sr. Quality Control Analyst Berlex Laboratories, Bothell, WA (July 2002-January 2003) Inspection, sampling, and testing of raw materials Tracking and coordination of raw material testing Generation and maintenance of raw material specifications and test methods Review of raw material assays Review, approval, and disposition of raw material test lots Raw Materials representative for weekly Leukine Production Team meetings Revision of QC Raw Materials policy SOPs Development, management and use of databases for tracking raw material testing. |
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| Quality Control Analyst I/II Immunex, Bothell, WA (August 1999-July 2002) Approval and qualification of raw materials for ENBREL Review and approval of raw material test lots Creation and maintenance of vendor information (certification, test methods, animal origin information, etc.) Tracking and coordination of raw material testing Method development and validation of an HPLC method for the quantitative analysis of complex amino acid formulations Review of raw material assays Generation and maintenance of raw material specifications and test methods Inspection, sampling, and testing of raw materials Tracking and maintenance of raw material stability samples and storage |
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| Laboratory Aid/Intern Fred Hutchinson Cancer Research Center, Seattle, WA (October 1997-August 1999) Sub-cloning and protein expression in E. Coli systems Purification, crystallization, and modeling of homing endonuclease/DNA substrate complexes Media preparation |
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| Student Trainee Douglas County Public Utility District No. 1, East Wenatchee, WA, (1995-1996) Preparation of reports and databases using MS Access, Excel, Word, and Project Execution of a survey to determine an optimum recreational usage policy for District park properties. Drafting of various electrical and structural schematics using AutoCAD. |
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| Publications: | |
| Galburt, E., Jurica, M., Chevalier, B., Erho, D. Tang, W.,
Monnat, R. J. Jr., and Stoddard, B.L. (2000) "Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site" J. Mol. Biol. 300 (4): p 877-887. |
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| Activities: | |
| Missionary in Russia Calvary Chapel, Vladimir, Russia (January 2003-present) Director of Sunday School ministry Teaching Christian History to youth group Leading a weekly Bible study Teaching science to elementary school children Completion of a Missions Training Program Studying the Russian Language Providing miscelaneous assistance for the Church (equipment set-up, ushering, running wide-screen projector, etc.) |
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| Short-term Missions Worker in Russia, Costa Rica, Guatemala,
and Mexico- Aid for orphanages, construction of educational facilities, small scale food distribution |
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