Danielle Macario
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Status:
4th Year Graduate Student- Biomedical Engineering
Focus:
Tissue Engineering
Advisor:
Gary Nackman, MD
Undergraduate College:
Immaculata University
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Research:
Investigating the mechanism behind shear stress induced detachment from biomaterials
Human endothelial cells fail to ultimately cover artificial vascular grafts. Previously published work by others has identified that shear stress can "rescue" endothelial cells from apoptosis, utilizing a model in which apoptosis is first induced by serum starvation. We question the relevance of this model. It is not yet clearly defined how shear stress induces cell detachment from their biomaterials. Two pathways are suspected, either healthy non-apoptotic endothelial cells are forcibly removed from the surface or mechanical forces such as shear stress and pressure induce apoptosis of the cells leading to altered morphology followed by detachment. We hypothesized that shear stress can induce apoptosis of a stable monolayer of endothelial cells adherent to an artificial biomaterial. Apoptosis would then lead to further detachment of the cells from their matrix. In our studies, detachment was observed concurrently with apoptosis of endothelial cells exposed to shear stress. We were succesfully able to inhibit cell detachment by inhibiting apoptosis using a pan-caspase inhibitor, Z-VAD-fmk. The molecular mechanism initiating apoptosis of the endothelial cells is suspected being at least partially due to activation of the death receptors, specifically the Fas receptor. Further investigation will reveal the molecular mechanisms and pathways involved in this process.
Publications & Presentations:
Danielle Macario B.A., Ildiko Entersz, Joachim Kohn Ph.D., Gary Nackman M.D. "PEG and Iodine Within Polycarbonates Antagonistically Affect Protein Adsorption"The 8th New Jersey Symposium on Biomaterials Science. Hyatt Regency Hotel, New Brunswick, NJ, November 8-10, 2006 (Poster)
Danielle K. Macario, B.A., Ildiko Entersz, Jean-Paul Abboud, B.S., and Gary B. Nackman, M.D. "Inhibition of Apoptosis Prevents Shear Induced Detachment of Endothelial Cells" Journal of Surgical Research. 2006 (Manuscript- Revising, Pending acceptance)
Danielle Macario B.A., Ildiko Entersz, Joachim Kohn Ph.D., Gary Nackman M.D. "PEG and Iodine Within Polycarbonates Antagonistically Affect Protein Adsorption" 1st Annual Methods in Bioengineering. July 17-18, 2006 (Poster)
Macario D.K., Entersz I., Bolikal D., Kohn J., Nackman G.B. "Interaction of Endothelial and Smooth Muscle Cells with Radiopaque, Resorbable Polymers" Association for Academic Surgery: First Academic Surgical Congress February 7-10, 2006 (Abstract Submitted)
Macario, D., Entersz I., Nackman GB. "Inhibition of Caspase Activity Prevents Shear-Induced Detachment of Endothelail Cells" Rutgers University, Fifth Annual Senior Design Conference for Biomedical Engineering. April 21, 2005 (Poster)
Macario, D., Entersz I., Bolikal, D., Kohn, J., Nackman GB. "Iodine Negates the Inhibitory Effect of PEG on Vascular Cells in a Resorbable Polymer" Rutgers University, Fifth Annual Senior Design Conference for Biomedical Engineering. April 21, 2005 (Poster)
Macario, D., Entersz I., Abboud JP, & Nackman GB. "Inhibition of Apoptosis Prevents Shear Induced Detachment of Endothelial Cells" 38th Annual Meeting of the Association for Academic Surgery November 11-13, 2004 (Abstract and Oral Presenation)
Macario, D., Entersz, I., Nackman, G. "SHEAR STRESS INDUCES APOPOTOSIS OF ENDOTHELIAL CELLS" Association for Academic Surgery Conference. Sacramento, November 12-15 2003 (Abstract and Poster)
Research Day Finalist "Shear Stress Induces Apoptosis in Endothelial Cells on Biomaterial" Macario, D. K., Entersz, I., Nackman, G. B.; Department of Surgery, UMDNJ-RWJMS, New Brunswick, NJ. (Poster) 6th Annual Research Day, November 15, 2004
Volunteering
28th Annual International Conference, IEEE Entineering in Medicine and Biology Society (EMBS). New York City, NY, USA, August 30- September 3, 2006
The 8th New Jersey Symposium on Biomaterials Science. Hyatt Regency Hotel, New Brunswick, NJ, November 8-10, 2006
NYAS? Careers in Engineering. NYU, New York City, NY, Month day, 2005
Awards/Honors
Honorable Mention from the NSF for a graduate research fellowship application
Who's Who Among Students in American Universities and Colleges
National Honors Society
Sigma Zeta Honors Society
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