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| Veterinary Immunology and Immunopathology Volume 58, Issues 3-4 , 19 September 1997, Pages 355-362 |
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| View Full Text in PDF (549 K) | |||||||||||||||
| Copyright � 1997 Published by Elsevier Science B.V. | |||||||||||||||
| Whole blood assay for examining lymphocyte blastogenesis of percichthyid bass (Morone) mrr01 | |||||||||||||||
| Chengjie Wang a, 1, Edward J. Noga a, Ramy Avtalion b and Michael G. Levy a a College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 27606, USA b Department of Life Sciences, Bar Ilan University, 52 100, Ramat Gan, Israel Received 26 September 1996; accepted 17 March 1997. Available online 13 February 1998. |
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| Abstract | |||||||||||||||
| A simple and reproducible method was developed for the measurement of blastogenesis of peripheral blood lymphocytes using whole blood of hybrid bass (striped bass [Morone saxatilis] female � white bass [M. chrysops] male) stimulated with Concanavalin A, phytohemagglutinin-P, lipopolysaccharide or pokeweed mitogen. Compared to traditional methods which use leucocyte separation procedures, whole blood culture is faster and less expensive. Only small aliquots of blood (10 ul per culture well) were needed, which would be beneficial for sampling small fish as well as for taking multiple samples from single animals. Optimal culture conditions for hybrid bass, including mitogen concentration, incubation temperature and incubation period, were determined. This is the first report to demonstrate a blastogenic response of whole blood cells in fish. | |||||||||||||||
| Author Keywords: Bass; Blastogenesis; Fish immunity; Mitogen; Whole blood lymphocyte culture | |||||||||||||||
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| Corresponding author. Tel.: +1 919 829 4236; fax: +1 919 829 4336 | |||||||||||||||
| 1 Present address: 7230 Hunter's Spring Drive, Charlotte, NC 28269 USA. | |||||||||||||||