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Research Experience
1. At the University of Oviedo, Spain (1993-1997):
1.a. Ribosomal proteins of Streptomyces (1993–1997). My PhD Thesis. Regulation of the expression of ribosomal protein genes, in relation to cell differentiation processes occurring during the Streptomyces life cycle. Cloning, sequencing and transcriptional analysis of two operons of ribosomal protein genes from S. coelicolor. Supervisors: Jose A. Salas and Gloria Blanco.
1.b. Biosynthesis of mithramycin in Streptomyces argillaceus (1996-1997). Identification of genes coding for glycosyltransferases and methyltransferases involved in the biosynthesis of mithramycin, an aromatic polyketide with antitumor and antimicrobial activities. Supervisors: Jose A. Salas and Carmen Mendez.
2. At the University of California, Davis, USA (1997-2000):
Biosynthesis of bleomycin in Streptomyces verticillus. Cloning and sequencing of the gene cluster responsible for biosynthesis of bleomycin, an antitumor antibiotic with a peptide-polyketide hybrid structure. Biochemical characterization of adenylation domains in non-ribosomal peptide synthetases. Genetic identification and biochemical characterization of a phosphopantetheinyl transferase from S. verticillus (this class of enzymes are responsible for post-translational modification of enzymes involved in the biosynthesis of fatty acids, polyketides and non-ribosomal peptides). P.I.: Ben Shen.
3. Back at the University of Oviedo, Spain (2000-2006):
3.a. Biosynthesis of borrelidin in Streptomyces rochei (2000). Identification of the gene cluster involved in the biosynthesis of borrelidin, a macrolide polyketide possessing antimicrobial, antitumor and anti-angiogenic activities. P.I.: Jose A. Salas.
3.b. Biosynthesis of indolocarbazoles in actinomycetes (2000-2006). Identification of the complete gene clusters for biosynthesis of rebeccamycin in Lechevalieria aerocolonigenes and for biosynthesis of staurosporine in Streptomyces longisporoflavus. Gene functions were analyzed by expression of the complete gene clusters and of selected gene subsets in an actinomycete host. These experiments resulted in the generation of recombinant strains that produced rebeccamycin, staurosporine and several indolocarbazole derivatives. Moreover, the expression in these recombinant strains of additional genes isolated from other microorganisms increased the number of derivatives produced by this approach (the additional genes were involved in the biosynthesis of metabolites that were structurally related to indolocarbazoles). Some studies were also done on the biosynthesis of violacein, a structurally-related pigment from Chromobacterium violaceum, a Gram-negative bacterium. P.I.: Jose A. Salas.
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Copyright © 2007, Cesar Sanchez. |
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CESAR SANCHEZ |
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Research Experience |