The regiment defended Yorktown, and was present at Williamsburg, but its first major battle was at Seven Pines, where it lost 150 men. It then took part in the Seven Days Battles, losing its colonel at Malvern Hill. Missing the Second Manassas, the Twenty-eighth fought at South Mountain and Antietam, held a supporting position at Fredricksburg, and was engaged at Chancellorsville.
By August 1863 the Georgians were in Charleston, and helped garrison Fort Wagner and Greg on Morris Island, and later Fort Johnson and Fort Sumter. In February 1864 the Twenty-eighth fought at Olustee under the command of Captain James W. Banning. Durning the battle Captain James Rowe of Company E "planted the colors of the regiment over two Nepoleon guns captured from the enmy." The Twenty-eighth lost ninety-five (ten killed and eighty-five wounded) men in the battle.
In the spring of 1864 the regiment returned to the Charleston defenses with the rest of Colquitt"s Brigades. When the brigade was ordered to Viginia the Twenty-eighth remained in Charleston on provost duty for several days. When it reached Virginia it was temporarily assigned to Martins's Brigade ,although it shortly thereafter rejoined Colquitt's command. The regiment then fought at Cold Harbor and Petersburg before being assigned to North Carolina, where it surrendered in 1865.
