Preparation of calibration curve 1. Using burette filled with water, prepare 6 sucrose solution of different concentration, namely 0.01M, 0.008M, 0.006M, 0.002M, 0.004M in test tube 2. Set up the polarimeter. 3. Pour 0.01M solution into measuring cylinder to measure volume of 50cm^3. 4. Use 50cm^3 solutions to test its angle of rotation. 5. Record the results. Repeat the above procedure with different concentration of sucrose. 6. Draw a calibration curve and find out the best-fit formula of it. We will use the best fit line to find out concentration of sucrose of soft drink. Test the pH of soft drink 1. Pour pH = 7 buffer into a beaker. 2. Calibrate the pH meter to 7 with pH = 7 buffer. 3. Rince the pH meter with deionized water. 4. Open the can of soft drink and pour it into the beaker. 5. Dip the pH meter into the soft drink immediately and record the result. Rince the pH meter with deionized water. 6. Repeat step 1-5 with different soft drinks. Remove CO2 and acid in soft drink 1. Pour 60cm3 volume of 0.1 M standard NaOH into beaker of soft drink with volume marked on the can to neutralize acid to stop hydrolysis. 2. Record the volume of soft drink using measuring cylinder. Loosely speaking, the volume of solution is the sum of volume of NaOH and soft drink volume. 3. Repeat the process with different soft drink. Test the sucrose content of soft drink 1. Pour 50cm^3 sample of soft drink into large tube using measuring cylinder. 2. Place the tube into polarimeter. 3. Adjust the polarized so that the light intensity seen from top is the greatest. 4. Record the angle of rotation of this soft drink. 5. Repeat the experiment with different soft drinks. 6. Calculate the sucrose concentration of soft drink.