What I learned about Tissue Culturing

  1. You must pay a lot of attention to your cells.  Feed them every other day.  Don't feed them cold food.
  2. Keep cells warm by placing them in an incubator.
  3. Clone them... uh, make sure they have lots of food for them to divide and make more of themselves.
  4. Treat cells like your baby.
  5. However, do not keep them as pets.
  6. Once you use (but, try not to abuse) them, lose them.
  7. Crowded cells are scientifically called confluent cells.
  8. To keep a cell line you must turn them into freezy pops at a certain point.
  9. Cells live and swim in their food.  They also poo in their food.
  10. When they soil their food too much they may die from their disgusting waste.
  11. Dead cells don't attach to the Petri.  Their dead floating carcass is rounded and dense.
  12. Some cell lines are "immortalized."  That doesn't actually mean they won't die.
  13. Sometimes you have to fuse cells with something else before they become immortals.
  14. You tell people you're going to feed your cells, but actually you're killing most of them.
    You keep only like 1/4 or sometimes as much as 1/2 of the cells, while sending the rest to their doom. Its quite funny.

 

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