1.
Prepare a wet
mount slide of a potato. Use a razor blade to slice a piece of tissue, as
thin as possible, from a potato. Be careful not to cut your fingers. Prepare a
wet‑mount slide; use a drop of water.
2.
Study the slide at low power (10X objective) and then at high power
(40X objective). Add a drop of Lugol's solution (I2KI) to the side
of the coverslip and observe the cells as the iodine solution makes contact
with them.
Make a sketch of the stained
structures!
Specimen:
________________________
Magnification: ______________________
Shape and Description: ________________
_______________________________
The small oval‑shaped blue‑black
structures are leucoplasts. Why did they turn blue?
How does the reaction of iodine with the potato cells
compare with what you observed in your onion epidermis preparation?
What
does this tell you about the differences between the storage products in onions
and potatoes?
Do you see any chloroplasts? Why or why not?
3. Prepare a wet mount slide of carrot or red pepper.
Use a razor blade to slice a piece of tissue, as thin as possible, from the
outer portion of a peeled carrot, or scrape some tissue from a red pepper.
Prepare a wet‑mount slide using water.
Can you see the
chromoplasts? Sketch them.
Specimen: ________________________
Magnification: ______________________
Shape and Description: ________________
_______________________________
northonline.sccd.ctc.edu/plortz/bio101/Lab%2002%20-%20Cells.DOC