The Influence of Packing on Free Radical Yields in Solid State DNA: Film versus Lyophilized Frozen Solution.Michael T. Milano and William A. BernhardAbstract |
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Radiation induced free radical damage in solid state DNA arises from direct-type damage mechanisms. In the present study, free radical yields (FRYs) in film and lyophilized Na DNA model systems are compared. The FRYs in lyophilized samples are significantly greater than those in films. Since DNA conformation cannot account for the differences in FRYs between different DNA preparations, it is proposed that the intermolecular spacing of DNA is a critical variable. The differences in the hydration dependence of FRYs between the film and lyophilized DNA model systems are consistent with this thesis.
The relatively large FRYs observed in lyophilized DNA emphasizes the fact that DNA is an extremely effective electron and hole scavenger, more so than previously thought.
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