Lab Tech

5/11/99

Blood

• Fill a tube w/blood and centrifuge, all the RBC's would be down at the bottom, red in color
• Stuff on top is clear to straw colored—plasma
• Things big enough to fall to bottom (45%)—RBC, WBC (right on top of the RBC's), platelets (line on top of the pellet)
• RBC's are the heaviest (do to iron)—1000x's more RBC's than WBC's
• WBC's are the biggest
• % of RBC in blood--hematocrit
• Plasma—55%
• 7% proteins
• 1% small molecules—E, testosterone,
• 1% electrolytes

Proteins

• 60% albumin—osmotic regulator, controls blood volume, carrier of things that are usually not soluble
• 36% globulins
• a --carrier of things that are usually not soluble ex: cholesterol
• b --carrier of things that are usually not soluble
• g --antibodies (immunnoglobulins)

• electrophoresis (gel)—gel w/ current running through it to separate molecules by weight
• 4% fibrinogen—forms clots
• serum proteins
• 63% albumin
• 37% globulins
• has no fibrinogen