The influence of proteinic fraction on electrophoresis and concomitant quantitative measurement of immunoglobulins in patients with multiple sclerosis

DROSSO TH,KOUNTOURIS D, KOSSIONI E,LIAPIS A, SIMA A AND SPAKOURIS D.

Internal Medicine and Microbiology, Pammakaristos Hospital Iacovaton 43 Athens 11144 and Medical Neurological Center, Michalakopoulou 45, Athens, Greece.

This study tries to test an observation with multiple sclerosis (MS) in electrophoresis of proteins and evaluates if this influence contributes in disease diagnosis. A quantitative measurement of immmunoglobulins IgA, IgG, and IgM was concomitantly performed. Eight-six patients with known MS aged 17-56 participated in this study. Proteinic electrophoresis was performed on acetic cellulin Helena and the quantitative assay of Iga, IgG and IgM with Turbi Tissue System-Behring.

Table 1.

Variable	Mean	Minimum	Maximum	s.d	Normal limits
Albumin	54.41	46	62	3.93	52.3-66
a1	3.155	2.2	4.5	0.43	3.3-7
a2	11.527	0.3	16.3	1.77	6.3-11.7
�	14.167	7.8	19.9	2.22	7.8-14.3
�	16.176	9.4	30.2	3.18	11.1-20.4
IgA	1061.2	650	1940	215.54	80-01700
IgG	209.19	60.9	463	88.57	85-450
IgM	179.72	19	874	132.52	60-370

A decrease in a₁ globulin was observed. We have some clinical evidence that patients with MS with a low a₁ globulin have a grave clinical picture. This observation has to be further tested. There is no influence on quantitative immunoglobulins `assay.

Abstract published in EUROPEAN JOURNAL ON NEUROLOGY 1996;3(5)

papaer presented at the 2^nd Congress of the European Federation of Neurological Societies, Rome, Italy, 30 October -3 November 1996.