Gamma Sigma Chapter was established as the first graduate chapter in January, 1922, and Beta Psi Chapter was organized in June, 1945. In 1959 both graduate chapters dropped the Greek nomenclature and united to become the Cincinnati Alumnae Chapter.

Throughout the years, Cincinnati sorors have been involved in the National Organization. The sorors entered activities of the Grand Chapter as they attended the Third National Convention in Philadelphia, Pennsylvania, in 1921. Ida Mae Rhodes became the first and only national Representative, as the office was abolished when sectional vice presidents were established. Ethel LaMay Calimese was elected treasurer during the same convention, and Martha Hall Ross became the Second Vice President in 1922.

In 1926 Zeta and Gamma Sigma Chapters hosted the Sixth National Convention in Cincinnati. Ethel LaMay Calimese was elected Grand President and Beatrice Morton was elected Secretary. In 1939 Elsie Austui became the eighth Grand President and Reber S. Cann, who helped establish the National Headquarters in Washington, D.C. served as National Secretary from 1944-1952, and National First Vice President from 1951-1954.