AM 05/I.00 INDEX NUMBER:_________________________
MATRICULATION AND SECONDARY EDUCATION CERTIFICATE EXAMINATIONS BOARD
UNIVERSITY OF MALTA, MSIDA
MATRICULATION CERTIFICATE EXAMINATION
ADVANCED LEVEL
MAY SESSION 2000
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Subject Title |
BIOLOGY |
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Paper No./Title |
Paper 1 |
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Date |
25th May 2000 |
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Time |
9.00 a.m. to 12 noon |
Directions to Candidates
For examiners’ use only:
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OPTIONS |
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Question |
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Total |
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Score |
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Maximum |
10 |
10 |
10 |
8 |
12 |
10 |
10 |
10 |
10 |
10 |
10 |
10 |
100 |
SECTION A
(Answer all questions in this section):
Figure 1: surface area of wetland colonised by Phragmites australis over time.
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[one mark] |
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[two marks] |
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[one mark] |
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[two marks] |
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[two marks] |
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1. |
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[two marks] [total: ten marks] |

Figure 2: Relationship between birth weight and degree of survival in female births (adapted from Karn, M.N. and L.S. Penrose (1951). Birth weight and gestation time in relation to maternal age, parity and infant survival. Ann. Eugenics, 161, 147-164.)
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[two marks] |
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[one mark] |
[continued on next page]
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[three marks] |

Figure 3: Selection for high and low oil content in corn kernels. (adapted from Dudley, J.W. (1977). Seventy-six generations of selection for oil and protein percentages in maize. In Proceedings of the International Conference on Quantitative Genetics. Pollack, E. O. Kempthorne and T.B. Baiely, Jr. [eds.]. Iowa State University Press, Ames, pp. 459-473.)
Figure 3 shows selection for high and low oil content in oil kernels in an experiment started in 1896 at the University of Illinois and continuing to the present. Selection for high oil content still continues to yield increases, whereas selection for low oil content has tapered to the 0 percent lower limit.
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[one mark] |
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[one mark] |
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[two marks] [total: ten marks] |
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A |
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D |
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Figure 4: Cell cycle from root tip cells in Allium. From Shaw, A.C., S.K. Lazell & G.N. Foster (1965). Photomicrographs of the flowering plant. Longman. |
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[one mark] |
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[two marks] |
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Stage A |
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Stage B |
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Stage C |
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Stage D |
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[two marks] |
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Cells from a shoot apical meristem |
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Microsporocytes within the microsporangia of plants |
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[two marks] |
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1. |
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2. |
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[three marks] [total: ten marks] |
Platyhelminthes, Crustacea, Annelida, Chlorophyta, Amphibia, Bryophyta, Echinodermata, Cnidaria
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Description |
Taxon |
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Chlorophylls a and b are the main photosynthetic pigments. |
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Life cycle shows alternation of generations, with the gametophyte as the dominant phase. |
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Sheds its exoskeleton by ecdysis; it is characterised by possession of a carapace or cephalothorax; lacks pedipalps. |
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Posseses a coelomic water vascular system, externally evident as muscular podia. |
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Dorsoventrally flattened acoelomates; cephalised; gut with multiple blind-ending branches |
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Animals displaying radial symmetry; the only body cavity is referred to as a coelenteron. |
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Semi-terrestrial vertebrates; larval stages have gills, which are usually lost in the adult. |
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Animal group where both internal and external anatomy exhibit metameric segmentation. |
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[one mark for each correct answer] [total: eight marks] |
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Figure 5: The Human Eye
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Label |
Structure |
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A |
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B |
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C |
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D |
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E |
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F |
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G |
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H |
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I |
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J |
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[one half-mark for each correct answer] [five marks] |
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Structure |
Function |
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A |
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E |
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I |
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J |
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[four marks] |
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[one mark] |
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[two marks] [total: twelve marks] |
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[two marks] |
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[four marks] |
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[two marks] |
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[two marks] [total: ten marks] |
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Advantage: |
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Disadvantage: |
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[two marks] |
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[three marks] |
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1. |
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2. |
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3. |
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4. |
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[one half-mark for each correct answer] [two marks] |
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Form |
Definition |
Organism |
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Binary fission |
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Budding |
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Fragmentation |
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[one half-mark for each correct answer] [three marks] [total: ten marks] |

Figure 6: Single mitochondrion from zymogen cell of mouse fundic gland. From: Helander, H. (1962). J. Ultrastruc. Research. Supplement 4.
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Structure A: |
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Structure B: |
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Structure C: |
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[three marks] |
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[two marks] |
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[three marks] |
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[one mark] |
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[one mark] [total: ten marks] |
D
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[two marks] |
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[two marks] |
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[three marks] |
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[three marks] [total: ten marks] |
SECTION B
(Answer one question from this section):OPTION 1: Biotechnology

Figure 7: Typical gene cloning experiment. From: Ketchum, P.A. (1988). Microbiology: Concepts and Applications. Wiley.
The diagram in Figure 7 (previous page) shows a typical gene-cloning experiment. Purified foreign DNA is inserted into a plasmid vector after both are treated with the same restriction endonuclease. These treated DNAs have complementing sticky ends that anneal to form a recombinant DNA. After nicks in the DNA are sealed by DNA ligase, the recombinant DNA is taken into a cell by artificial transformation. The foreign genes are cloned in this cell when multiple copies of the plasmid are made.
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[two marks] |
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[two marks] |
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[two marks] |
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[two marks] |
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[two marks] [total: ten marks] |
Option 2: The Diversity of Eukaryotes

Figure 8: Euglena. Adapted from Brusca, R.C. and G.J. Brusca (1990). Invertebrates. Sinauer Associates Inc.
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Label |
Structure |
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A |
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C |
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D |
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E |
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F |
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G |
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[one half-mark for each correct answer] [three marks] |
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[one mark] |
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1. |
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2. |
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[two marks] |
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Structure |
Function |
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B |
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D |
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F |
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[four marks] [total: ten marks] |
Option 3: Topics in Applied Botany
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[two marks] |
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1. |
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2. |
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3. |
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4. |
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[one half-mark for each correct answer] [two marks] |
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[two marks] |
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[two marks] |
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Advantage: |
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Disadvantage: |
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[one mark] |
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Advantage: |
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Disadvantage: |
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[one mark] [total: ten marks] |