BIO 306: CELL BIOLOGY
LAB EXPERIMENT EQUIPMENT/TECHNOLOGY EMPLOYED
1 Diffusion, Osmosis, and the functional Construction of Osmometer, Dialysis
significance of biological membranes
2. Dialysis-Tests for Chloride ions and Starch Dialysis equipment
3. Hydrolysis of protein and seperation Chromatography
of amino acid by paper chromatography
4. Protein purification by coloumn Chromatography
chromatography & Dialysis
5. Spectrophotometric determination Spectrophotometry
of absorption spectrum of bio-
molecules:Chloroplast, chromoplast etc
6. Effect of substrate concentrations, pH and Enzymology
temperature on enzyme activity
7 SDS-polyacrilamide Gel Electrophoretic Electrophoresis
seperation of proteins