Stock A (can be kept up to 3 months at 4oC)
|
NaCl |
5.534 g |
11.068 g |
|
KCl |
0.356 g |
0.712 g |
|
KH2PO4 |
0.162 g |
0.324 g |
|
MgSO4 anhydrous |
0.143 g |
0.286 g |
|
Glucose |
1.000 g |
2.000 g |
|
Sodium lactate |
2.610 g |
5.220 g |
|
H2O |
To 100 ml |
To 200 ml |
Stock B (prepared freshly)
|
NaHCO3 |
0.210 g |
0.420 g |
|
Phenol red |
0.001 g |
0.002 g |
|
H2O |
10 ml |
20 ml |
Stock C (prepared freshly)
|
Pyruvate (100mM) |
10 ul |
50 ul |
0.15 ml |
|
H2O |
290 ul |
1.45 ml |
4.35 ml |
Stock D (prepared freshly)
|
CaCl2.2H2O |
0.252 g |
0.504 g |
|
H2O |
10 ml |
20 ml |
Stock E (can be kept up to 3 months at 4oC)
|
HEPES |
2.929 g |
5.958 g |
|
Phenol red |
0.005 g |
0.010 g |
|
H2O |
50 ml |
100 ml |
Adjust pH to 7.4 with 4 M NaOH
M2
|
|
50 ml |
100 ml |
150 ml |
200 ml |
300 ml |
|
A |
5.0 |
10.0 |
15.0 |
20.0 |
30.0 |
|
B |
0.8 |
1.6 |
2.4 |
3.2 |
4.8 |
|
C |
0.5 |
1.0 |
1.5 |
2.0 |
3.0 |
|
D |
0.5 |
1.0 |
1.5 |
2.0 |
3.0 |
|
E |
4.2 |
8.4 |
12.6 |
16.8 |
25.2 |
|
H2O |
39.0 |
78.0 |
117.0 |
156.0 |
234.0 |
|
BSA |
200 mg |
400 mg |
600 mg |
800 mg |
1.2 g |
|
P/S |
0.5 |
1 |
1.5 |
2.0 |
3.0 |
M16
|
|
10 ml |
20 ml |
40 ml |
50 ml |
|
A |
1.0 |
2.0 |
4.0 |
5.0 |
|
B |
1.0 |
2.0 |
4.0 |
5.0 |
|
C |
0.1 |
0.2 |
0.4 |
0.5 |
|
D |
0.1 |
0.2 |
0.4 |
0.5 |
|
H2O |
7.8 |
15.6 |
31.2 |
39.0 |
|
BSA |
40 mg |
80 mg |
160 mg |
200 mg |
|
P/S |
0.1 |
0.2 |
0.4 |
0.5 |