A, Glass powder. (what glass?)
1, Mix 10ml glass in 20ml H2O, stir well.
2, Settle for 1 hour, discard the settle (coarse) powder, spin to recover the fine.
3, Resuspend in 8ml H2O and 8ml conc. Nitric Acid.
4, Boil in hood, cool down, spin down and resuspend to wash away the acid.
5, Store as 50% slurry.
B, 6M NaI solution.
90.8g NaI
0.5gm NaSO
Dissolve slowly in water to a final volume of 100ml. Filtered, then add back a few crystal
of sodium sulfide. Store at 4oC and wrapped in aluminum foil to protect
from light.
C, NEW Buffer
| Final conc. | Stock Conc. | Dil. Factor | For 50ml | For 10ml | |
| EtOH | 50% | 100% | 2 | 25 | 50 |
| NaCl | 0.1M | 5M | 50 | 1 | 2 |
| Tris pH7.5 | 10mM | 1M | 0.5 | 0.5 | 0.51 |
| EDTA | 1mM | 0.5M | 500 | 0.1 | 0.2 |