Giardia lamblia trophozoit stained with acridine orange.
The two nuclei and several small vesicles in the periphery of the cytoplasm are brightly fluorescent.
See also Elena Hilario's slides from her Ph.D. defense

Transgenic (left) and control (right) carrot plants.
The carrot on the left expresses an antisense mRNA against the vacuolar
ATPase catalytic subunit. The expression is driven by a strong constitutive promoter. The transgenic plants have a lower level of V-ATPases on their vacuolar membrane, but unchanged V-ATPase levels on other endomembranes. The antisense expressing plants have larger cells, an altered leaf morphology, and they are more sensitive to water stress. For further information see Gogarten, J.P. , Fichmann, J., Braun, Y., Morgan, L., Styles, P.,Taiz, S. L., Delapp,K., Taiz,L. (1992): The Use of Antisense mRNA to Inhibit the Tonoplast ATPase in Carrot. The Plant Cell 4, 851-864.

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